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  • Name: Ravi A.
  • Lab Group: Cairns lab
  • Date: 13Sept2011
  • Request ID: 8341
    • Cell Type: H1 and hNPC
    • Ab: TFIIIC
  • Shear Method: Manual Sonnicator
    • Cells/ml:
    • Shear Time: 20X of 30 seconds ON and 40 second OFF
    • Power Setting: 3-4
    • Input size range on gel: 200-600 bp
  • IP Conditions
    • IP Kit: Home brew
    • IP protocol: Lab protocol
    • Cleanup of IP: Qiagen PCR cleanup
    • qPCR: 4-10X enrichment
  • Re-fragment: Bioruptor
    • Shear time: 15 minutes of 30 seconds ON and 30 seconds OFF
    • Shear power:
  • Library prep: TruSeq RNA sample prep kit
    • PCR cycles: 10 cycles
    • PCR enzyme: Phusion polymerase
  • Illumina Sequencing
    • Number of reads per sample:
    • Number of mapped reads
    • Enriched sites: 1500 regions in H1_TFIIIC ChIP (FDR 0.1%), 1100 regions in NPC_TFIIIC ChIP (FDR 1%)