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Biomolecular Nuclear Magnetic Resonance

Director
Jack Skalicky, PhD
skalicky@biochem.utah.edu
801-585-7363

The Biomolecular Nuclear Magnetic Resonance (BNMR) Shared Resource provides a broad range of NMR-based services for Huntsman Cancer Institute (HCI) members and the entire University of Utah research community. The Resource provides high-level technical support and access to state-of-the-art NMR software and hardware, including spectrometers operating at 400, 500, 600, 800, and 900 MHz for protein/nucleic acid structure determination as well as routine analytical NMR services. The first three instruments are located at the University of Utah in centralized space and the latter two instruments in Colorado-based regional system (Boulder, 800; Denver, 900). The 400 MHz spectrometer is dedicated to small molecule, organic, and natural products NMR applications. The 500 MHz and 600 MHz spectrometers were recently upgraded to increase BNMR Shared Resource capability. The 600 MHz spectrometer is the highest field spectrometer in Utah. The BNMR Shared Resource manages Sun, SGI, and LINUX workstations for data processing, analysis, and structure determination through the Colorado-based regional system. Commonly used software for NMR spectroscopy is provided, including CYANA2.1, FELIX2004, MOLMOL, NMRPIPE, PYMOL, SPARKY, VNMRJ, and XPLOR-NIH.

The BNMR Shared Resource is directed by Jack Skalicky, PhD, a Research Assistant Professor in Biochemistry, who provides technical support for the most demanding NMR projects and assists researchers with data collection, processing, and analysis. The Resource has a full-time NMR technician responsible for training new users, performing scheduled maintenance, and recording small-molecule NMR experiments on the 400 and 500 MHz spectrometers. A shared hardware technician provides repairs.

The Resource serves members of all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation, Cell Response and Regulation, and Experimental Therapeutics) to characterize molecules, particularly proteins, that play roles in cancer mechanisms. In addition, characterization of small compounds, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeutics activities of HCI.

For more information, please see the BioNMR webpage on the University of Utah HSC Core Facility website: http://www.cores.utah.edu/?page_id=3679.

Biomolecular Nuclear Magnetic Resonance Governance

Director
Jack Skalicky, PhD

HCI Senior Director Oversight
Bradley Cairns, PhD

Faculty Advisory Committee Members
Dana Carroll, PhD
Darrell Davis, PhD
David Goldenberg, PhD
Chris Ireland, PhD
John Phillips, PhD (ex officio)
Wesley Sundquist, PhD

If use of this resource results in a publication, please acknowledge the Cancer Center Support Grant by using the following text: "The project described was supported by Award Number P30CA042014 from the National Cancer Institute. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health."