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High Throughput Genomics

The High Throughput Genomics Shared Resource provides investigators at the University of Utah with access to Illumina sequencing (HiSeq and MiSeq), the Fluidigm C1 Single-Cell Auto Prep System and to the Affymetrix and Agilent Technologies microarrays platforms.  The Resource is operated as a full-service facility that provides expertise in experimental handling of all aspects of these technologies.  Services offered by the Resource include evaluation of nucleic acid sample quality; construction of Illumina sequencing libraries; sequencing of libraries on an Illumina HiSeq or MiSeq; labeling of microarray samples with modified nucleotides; microarray hybridization; microarray scanning; and feature extraction and annotation of data from scanned images.

These technologies provide investigators with a diverse set of tools to perform gene, exon, or miRNA expression analysis; SNP and DNA copy number profiling,; location analysis of DNA binding proteins; DNA methylation profiling; metagenomic  studies; , exome and whole genome sequencing and the enrichment and sequencing of targeted regions of the genome.   Instrumentation to support these applications include the following: two Illumina HiSeq instruments , one Illumina c-Bot, one Illumina MiSeq, a Fluidigm C1 Single-Cell Auto Prep System, a NanoDrop Spectrophotometer, an Agilent Technologies 2200 TapeStation, two Invitrogen Qubit 2.0 Fluorometers, one Agilent Technologies Microarray Scanner and two Microarray Hybridization Ovens, one Affymetrix Hybridization Oven 640 and a Fluidics Station 450, a Covaris Adaptive Focused Acoustics Model S2, an Agilent Technologies Bravo Liquid Handling System, a Bio-Rad CFX Connect Real Time System and seven Bio-Rad thermal cyclers.