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In Vivo Tumor Studies and biomarker identification

At the PRR, we house a centralized resource for animal models along with expertise in experimental design and technical skills. We have completed several types of projects, including tumor monitoring of knock-down cell lines vs. wild type and immune based projects. Our typical tumor-monitoring pipeline includes:

  • Tumor transplantation in an orthotopic or subcutaneous site.
    • Transplantation sites include mammary fat pad and intra-tibial; ask about additional orthotopic sites.
    • When appropriate, transplantation includes Matrigel or hormone pellets.
  • Tumor growth measurements
    • Tumors and body weight are measured twice weekly either by caliper or bioluminescence.
  • Necropsy to harvest tumor tissue and metastatic organs for histology and RNA/DNA analysis.
    • Collected based on experimental design.
    • Includes flash freezing and paraffin embedded tissue.
  • Additionally, we can provide several imaging techniques for quantifying tumor progression and metastasis including bioluminescent imaging, fluorescence imaging, CT scans of bone and lung, and brain MRI.

  • We also work in close collaboration with the Center for Quantitative Cancer Imaging and can provide real-time scans of glucose metabolism, mineralization of bone, cell proliferation, hypoxia, angiogenesis, and perfusion.

We can help in both consultation and executing all aspects of your in vivo experiments. We provide you with necropsy tissue, survival curves, and growth data, everything you need to generate a figure for publication in a paper or grant.